Journal: Scientific Reports

Article Title: Expression and significance of IL-6 and IL-8 in canine mammary gland tumors

doi: 10.1038/s41598-023-28389-3

Figure Lengend Snippet: Serum levels of IL-6, IL-8 and IL-10 in CMGTs and healthy dogs. The serum levels of IL-6 ( A ), IL-8 ( B ) and IL-10 ( C ) in CMGTs and healthy dogs were detected by ELISA assay. One-way analysis of variance (ANOVA) by Tukey’s multiple comparison test was used to analyze multiple group comparisons data. The results from three independent experiments are presented as the mean ± standard deviation (SD). *denotes P < 0.05 and **denotes P < 0.01 compared with the healthy canine mammary gland serum group.

Article Snippet: Immunohistochemical method (IHC) was performed on 3 μm thick sections with the following primary antibodies, such as: polyclonal rabbit anti-IL-6 (dilution, 1:200), polyclonal rabbit aniti-IL-8 (dilution, 1:200) and polyclonal rabbit anti-IL-10 (1:150) (Bioss Biotechnology, Beijing, China).

Techniques: Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Scientific Reports

Article Title: Expression and significance of IL-6 and IL-8 in canine mammary gland tumors

doi: 10.1038/s41598-023-28389-3

Figure Lengend Snippet: Immunohistochemical staining of IL-6, IL-8 and IL-10 in CMGTs and normal canine mammary gland tissues. Representative images (200X) show IHC staining of ( A , D and G ) IL-6, ( B , E and H ) IL-8 and ( C , F and I ) IL-10 in CMGTs and normal canine mammary gland tissue sections. Both cell membrane and cytoplasmic staining is indicated.

Article Snippet: Immunohistochemical method (IHC) was performed on 3 μm thick sections with the following primary antibodies, such as: polyclonal rabbit anti-IL-6 (dilution, 1:200), polyclonal rabbit aniti-IL-8 (dilution, 1:200) and polyclonal rabbit anti-IL-10 (1:150) (Bioss Biotechnology, Beijing, China).

Techniques: Immunohistochemical staining, Staining, Immunohistochemistry

Journal: Scientific Reports

Article Title: Expression and significance of IL-6 and IL-8 in canine mammary gland tumors

doi: 10.1038/s41598-023-28389-3

Figure Lengend Snippet: Expression of IL-6, IL-8 and IL-10 proteins in CMGTs and normal canine mammary gland tissues. ( A–D ) The levels of IL-6, IL-8 and IL-10 protein in CMGTs and normal canine mammary gland tissues were estimated by western blot analysis. β-actin was used as loading control. Uncropped images for Western blots are presented in Supplementary Fig. . Relative protein levels were quantified using ImageJ 1.48 software. Immunoblots were captured to calculate the normalized values presented in the graph by estimation of express levels from IL-6, IL-8 and IL-10 proteins relative to β-actin protein. Data are presented as mean ± SD of triplicate experiments. *denotes P < 0.05 and ** P < 0.01 compared with the healthy canine mammary gland tissues group.

Article Snippet: Immunohistochemical method (IHC) was performed on 3 μm thick sections with the following primary antibodies, such as: polyclonal rabbit anti-IL-6 (dilution, 1:200), polyclonal rabbit aniti-IL-8 (dilution, 1:200) and polyclonal rabbit anti-IL-10 (1:150) (Bioss Biotechnology, Beijing, China).

Techniques: Expressing, Western Blot, Software

Journal: Life

Article Title: Immunomodulatory Effect of Lactobacillus reuteri ( Limosilactobacillus reuteri ) and Its Exopolysaccharides Investigated on Epithelial Cell Line IPEC-J2 Challenged with Salmonella Typhimurium

doi: 10.3390/life12121955

Figure Lengend Snippet: Dot blot analysis of culture supernatants of each experimental group performed in triplicate, spotted onto nitrocellulose membrane for IL-8 detection. Supernatants from individual experimental groups expressing IL-8 on nitrocellulose membrane. Detection was performed with rabbit anti-IL-8 polyclonal antibody obtained from Antibodies-online GmbH, and with CF ® 770 goat anti-rabbit IgG conjugated antibody, obtained from Bio-Connect. The membranes were scanned in an Odyssey Scanner.

Article Snippet: The membranes were then incubated in 1% BSA in Tris-buffered saline containing Tween-20 (0.05%) containing rabbit anti-IL-8 polyclonal antibody (Antibodies-online GmbH, Germany) at a 1:300 dilution, for 1 h at room temperature.

Techniques: Dot Blot, Expressing